Antigen Processing & Presentation Using the HEL ProteinWe analyze the cellular and molecular events that transpire in APC when it takes up the protein HEL, and that results in the generation of a complex of processed peptide and class II-MHC molecules.
Autoimmune DiabetesThe major investigations center on examining processing and presentation of the antigens responsible for autoimmune diabetes, and the biology of the autoimmune CD4 T cells. We mostly examine the mutant mouse NOD that spontaneously develops autoimmune insulitis with diabetes.
The long-term goal of the laboratory is to understand antigen presentation, both at the cellular and biochemical level, and to correlate antigen presentation events to the response of CD4 T lymphocytes. Our focus is on biochemical and biological parameters related to antigen selection, recognition and processing by antigen presenting cells (APC). The laboratory combines biochemical analysis of peptide binding to class II-MHC molecules, examination of the MHC-peptidome from APC using mass spectrometry approaches, cell biological analysis of presentation, and the examination and biological responses of CD4 T cells directed to the selected proteins and peptides. LEARN MORE