Contact Us

Emil R. Unanue
Unanue@pathology.wustl.edu

 

Javier A. Carrero
carrero@pathology.wustl.edu

 

Physical Location
Barnes Jewish Children's Institute for Health, 8th Floor

Office Phone
(314) 747-0561 or (314) 362-7440

Lab Phone
(314) 362-8752

Fax
(314) 362-1403

Mailing Address
Washington University School of Medicine
Department of Pathology and Immunology
660 S. Euclid Avenue, Campus Box 8118
St. Louis, MO 63110 USA

 

The techniques used in the laboratory are available by writing to us at: unanue@wustl.edu. These include MHC-class II isolation, peptide-MHC binding, T cell assays, hybridoma development, peptide analysis, radioiodination, peptide isolation from MHC molecules, Listeria monocytogene handling, and others.

The B cell hybridoma producing antibodies to the 48-61 peptide of hen-egg white lysozyme bound to I-Ak is available at ATCC:
AW 3.18.14: ATCC number CRL-2826

The B cell hybridoma producing an antibody specific to the 3A9 clonotype (a T cell reactive to 48-61-I-Ak complex) is available at ATCC:
IG12: ATCC number CRL-2827

T cell hybridomas to various HEL and insulin peptides can be obtained by contacting us at our email address.

Most of the strains of mice that we use are available at the Jackson laboratory. Unique to us are:
i) two strains of B10.BR mice expressing lysozyme under the insulin promoter; details on them are given in Byersdorfer et al , J Immunol, 175:4347, 2005
ii) two strains bearing a T cell receptor transgene to lysozyme peptides; details are available in Lovitch et al, J Immunol, 178: 122, 2007
iii) a strain bearing a membrane form of lysozyme, under the E alpha promoter, described in Peterson et al, Immunity, 11:453, 1999

The strains of Listeria monocytogenes were obtained from either Pascale Cossart at the Pasteur Institute, who also developed, and provided us with, the monoclonal antibodies to listeriolysin-O used in Edelson et al, Immunity 14:503, 2001; or Daniel Portnoy at UC-Berkeley

A large list of peptides isolated from cells bearing I-Ak, I-Ag7, I-Ad and Kd can be obtained by writing to us.

Details of the mass spectrometry laboratory of Dr. Michael Gross can be found at: http://www.chemistry.wustl.edu/faculty/mgross

Our laboratory is supported by grants from:

Janssen Pharmaceuticals, a subsidiary of Johnson and Johnson.

The NIH; in particular, the National Institute of Allergy and Infectious Diseases; the National Institure of Diabetes and Digestive Diseases,

The Juvenile Diabetes Research Foundation,

The Kilo Diabetes and Vascular Research Foundation. 

Last revision: February 16, 2016

Graduate Program Affiliation: Immunology Program